Minor transcription initiation events indicate that both human mitochondrial promoters function bidirectionally.
نویسندگان
چکیده
منابع مشابه
Organization of the human mitochondrial transcription initiation complex
Initiation of transcription in human mitochondria involves two factors, TFAM and TFB2M, in addition to the mitochondrial RNA polymerase, POLRMT. We have investigated the organization of the human mitochondrial transcription initiation complex on the light-strand promoter (LSP) through solution X-ray scattering, electron microscopy (EM) and biochemical studies. Our EM results demonstrate a compa...
متن کاملInitiation of transcription from each of the two human mitochondrial promoters requires unique nucleotides at the transcriptional start sites.
Promoters for transcriptional initiation on each strand of human mtDNA lie in close proximity in the displacement-loop region. Transcription initiates within these promoter elements, providing an opportunity to analyze the effects of nucleotide sequence changes on the efficiency and fidelity of this event within two delimited regions. Each promoter was individually altered by a site-directed mu...
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Transcription initiation is a highly regulated step of gene expression. Here, we discuss the series of large conformational changes set in motion by initial specific binding of bacterial RNA polymerase (RNAP) to promoter DNA and their relevance for regulation. Bending and wrapping of the upstream duplex facilitates bending of the downstream duplex into the active site cleft, nucleating opening ...
متن کاملDistinct and Contrasting Transcription Initiation Patterns at Mycobacterium tuberculosis Promoters
Although sequencing of Mycobacterium tuberculosis genome lead to better understanding of transcription units and gene functions, interactions occurring during transcription initiation between RNA polymerase and promoters is yet to be elucidated. Different stages of transcription initiation include promoter specific binding of RNAP, isomerization, abortive initiation and promoter clearance. We h...
متن کاملHuman mitochondrial transcription factors TFAM and TFB2M work synergistically in promoter melting during transcription initiation
Human mitochondrial DNA is transcribed by POLRMT with the help of two initiation factors, TFAM and TFB2M. The current model postulates that the role of TFAM is to recruit POLRMT and TFB2M to melt the promoter. However, we show that TFAM has 'post-recruitment' roles in promoter melting and RNA synthesis, which were revealed by studying the pre-initiation steps of promoter binding, bending and me...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 1986
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.6.1.294